ABA-Induced Vegetative Diaspore Formation in Physcomitrella patens

Physcomitrella patens

Arabidopsis and other species, this work has also begun to reveal the levels of evolutionary conservation within the flowering plants and even outside the plant kingdom. What does the future hold for Antirrhinum research? In addition to its continuing role as a genetic model for plant biology and development, several new experimental avenues are beginning to be explored with Antirrhinum. A majo...

Gene targeting in Physcomitrella patens.

Gene-targeting efficiency in the land plant Physcomitrella patens (Bryophyta) can only be compared with that observed in Saccharomyces cerevisiae. Sequencing programs and microbiological molecular genetic approaches are now being developed to unravel the precise function of plant genes. Physcomitrella patens, as the new 'green yeast', might well become a major tool for functional genomic studie...

II intron in Physcomitrella patens

Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

The moss Physcomitrella patens is unique for the high frequency of homologous recombination, haploid state, and filamentous growth during early stages of the vegetative growth, which makes it an excellent model plant to study DNA damage responses. We used single cell gel electrophoresis (comet) assay to determine kinetics of response to Bleomycin induced DNA oxidative damage and single and doub...

Light- and dark-induced action potentials in Physcomitrella patens.

Glass microelectrodes were inserted into Physcomitrella patens gametophyte leaves and action potentials (APs) were recorded in response to sudden illumination as well as after darkening, i.e., when the dark-induced membrane depolarization crossed a threshold. Application of 5 mM La(3+) (a calcium channel inhibitor), 10 mM TEA(+) (a potassium channel inhibitor) and increased free Ca(2+) resulted...